Which cell type is least likely to be positive for Sudan Black B stain?

Which cell type is least likely to be positive for Sudan Black B stain?

• A. Granulocyte
• B. Lymphocyte
• C. Monocyte
• D. Erythrocyte

Answer: (B)

Sudan Black B stain is primarily used to detect lipids and phospholipids within cells, and it is particularly effective in identifying certain types of cells that contain these substances. Granulocytes, such as neutrophils, eosinophils, and basophils, are known to be positive for this stain due to the presence of lipid-rich granules in their cytoplasm. Monocytes also display a positive reaction because they contain some lipid components. Erythrocytes can occasionally show a reaction with Sudan Black B, but this is less common than with granulocytes and monocytes. In contrast, lymphocytes are generally the least likely to stain positively with Sudan Black B. This is primarily due to their smaller size and the absence of significant lipid content compared to the other cell types. Lymphocytes are more homogeneous and do not have the granules that would react with the stain, making them the least likely to yield a positive result in this context.

Sudan Black B stain is primarily used to detect lipids and phospholipids within cells, and it is particularly effective in identifying certain types of cells that contain these substances. Granulocytes, such as neutrophils, eosinophils, and basophils, are known to be positive for this stain due to the presence of lipid-rich granules in their cytoplasm. Monocytes also display a positive reaction because they contain some lipid components.

Erythrocytes can occasionally show a reaction with Sudan Black B, but this is less common than with granulocytes and monocytes. In contrast, lymphocytes are generally the least likely to stain positively with Sudan Black B. This is primarily due to their smaller size and the absence of significant lipid content compared to the other cell types. Lymphocytes are more homogeneous and do not have the granules that would react with the stain, making them the least likely to yield a positive result in this context.